验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, A549 cells, human lung tissue, human placenta tissue, mouse lung tissue, Raji cells |
| Positive IP detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12447-1-AP targets TBX6 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3050 Product name: Recombinant human TBX6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 87-436 aa of BC026031 Sequence: SLPGVSLSLENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRENGRNCKRERDARVKRKLRGPEPAATEAYGSGDTPGGPCDSTLGGDIRESDPEQAPAPGEATAAPAPLCGGPSAEAYLLHPAAFHGAPSHLPTRSPSFPEAPDSGRSAPYSAAFLELPHGSGGSGYPAAPPAVPFAPHFLQGGPFPLPYTAPGGYLDVGSKPMY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | T-box 6 |
| 别名 | DFNB67, T box 6, T box protein 6, TBX6 |
| 计算分子量 | 436 aa, 47 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC026031 |
| 基因名称 | TBX6 |
| Gene ID (NCBI) | 6911 |
| RRID | AB_2200836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TBX6 antibody 12447-1-AP | Download protocol |
| WB protocol for TBX6 antibody 12447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
