验证数据展示
经过测试的应用
| Positive WB detected in | CTLL-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83804-6-RR targets TBX21/T-bet in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | TBX21/T-bet fusion protein Ag4618 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | T-box 21 |
| 别名 | TBX21, TBX21,T-bet, TBX 21, T-box transcription factor TBX21, T-box protein 21 |
| 计算分子量 | 535 aa, 58 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC039739 |
| 基因名称 | TBX21/T-bet |
| Gene ID (NCBI) | 30009 |
| ENSEMBL Gene ID | ENSG00000073861 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9UL17 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX21/T-bet antibody 83804-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |