TBX21,T-bet Polyclonal antibody
TBX21,T-bet Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, mouse thymus tissue, RAW 264.7 cells |
Positive IP detected in | mouse thymus tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | NK92 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13700-1-AP targets TBX21,T-bet in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | TBX21,T-bet fusion protein Ag4618 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | T-box 21 |
Synonyms | T bet, T box 21, T box protein 21, T PET, TBET, TBLYM, TBX21, TBX21,T bet, TBX21,T-bet, Transcription factor TBLYM |
Calculated Molecular Weight | 535 aa, 58 kDa |
Observed Molecular Weight | 60-62 kDa |
GenBank Accession Number | BC039739 |
Gene Symbol | TBX21 |
Gene ID (NCBI) | 30009 |
ENSEMBL Gene ID | ENSG00000073861 |
RRID | AB_10666436 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UL17 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TBX21,T-bet antibody 13700-1-AP | Download protocol |
IHC protocol for TBX21,T-bet antibody 13700-1-AP | Download protocol |
IP protocol for TBX21,T-bet antibody 13700-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.
| ||
Int J Cardiol Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome. | ||
Mol Nutr Food Res A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways | ||
Biomed Pharmacother Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity. | ||
Front Pharmacol Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy. | ||
Front Pharmacol Cisplatin Promotes the Efficacy of Immune Checkpoint Inhibitor Therapy by Inducing Ferroptosis and Activating Neutrophils. |