验证数据展示
经过测试的应用
| Positive WB detected in | NK-92 cells, A549 cells |
| Positive IP detected in | NK-92 cells |
| Positive IF/ICC detected in | NK-92 cells |
| Positive FC (Intra) detected in | NK92 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13700-1-AP targets TBX21/T-bet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4618 Product name: Recombinant human TBX21,T-bet protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC039739 Sequence: MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | T-box 21 |
| 别名 | TBX21, TBX21,T-bet, T bet, T-box protein 21, T-box transcription factor TBX21 |
| 计算分子量 | 535 aa, 58 kDa |
| 观测分子量 | 60-62 kDa |
| GenBank蛋白编号 | BC039739 |
| 基因名称 | TBX21/T-bet |
| Gene ID (NCBI) | 30009 |
| ENSEMBL Gene ID | ENSG00000073861 |
| RRID | AB_10666436 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL17 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| IF protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| IP protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| WB protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.
| ||
Nat Immunol The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates | ||
Nat Immunol Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted CD8(+) T cell fate. | ||
Adv Sci (Weinh) DMAP1 Deficiency Suppresses Lung Cancer Progression by Destabilizing Replication Fork and Activating IFN Signaling-Mediated Anti-tumor Immunity | ||
Adv Sci (Weinh) Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation | ||
Adv Sci (Weinh) Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment |
