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验证数据展示

  • IF Staining of HeLa using 16930-1-AP

    IF Staining of HeLa using 16930-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16930-1-AP (TBX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human placenta using 16930-1-AP

    WB analysis of human placenta using 16930-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16930-1-AP (TBX2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16930-1-AP

    IHC staining of human kidney using 16930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16930-1-AP (TBX2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 16930-1-AP

    IHC staining of human kidney using 16930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16930-1-AP (TBX2 antibody) at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO验证

TBX2 Polyclonal antibody

TBX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16930-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

T-box transcription factor TBX2, T-box protein 2, T-box 2, T box protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, BxPC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16930-1-AP targets TBX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 TBX2 fusion protein Ag10393 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T-box 2
别名 T-box transcription factor TBX2, T-box protein 2, T-box 2, T box protein 2
计算分子量 702 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC052566
基因名称 TBX2
Gene ID (NCBI) 6909
RRIDAB_2200526
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13207
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TBX2 antibody 16930-1-APDownload protocol
IHC protocol for TBX2 antibody 16930-1-APDownload protocol
IF protocol for TBX2 antibody 16930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Chem

Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins.

Authors - Bianca Del B Sahm
  • KD Validated
View Article
humanWB

Int J Mol Sci

Proteomics Analysis of the Polyomavirus DNA Replication Initiation Complex Reveals Novel Functional Phosphorylated Residues and Associated Proteins

Authors - Rama Dey-Rao
View Article