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- KD/KO Validated
TBRG4 Polyclonal antibody
TBRG4 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | U2OS cells, Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : N/A |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
16245-1-AP targets TBRG4 in WB, IP, IF, IHC, ELISA, HITS-CLIP applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, HITS-CLIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | TBRG4 fusion protein Ag9176 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transforming growth factor beta regulator 4 |
| Synonyms | CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4 |
| Calculated Molecular Weight | 631 aa, 71 kDa |
| Observed Molecular Weight | 60 kDa, 71 kDa |
| GenBank Accession Number | BC017235 |
| Gene Symbol | TBRG4 |
| Gene ID (NCBI) | 9238 |
| RRID | AB_2271487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969Z0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| IHC protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| IF protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| IP protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Alliance Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins. |
