验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, pig cerebellum tissue, rat cerebellum tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
| IF | See 5 publications below |
产品信息
66564-1-Ig targets TBR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15262 Product name: Recombinant human TBR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-196 aa of BC104844 Sequence: MQLEHCLSPSIMLSKKFLNVSSSYPHSGGSELVLHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDSPGDVQRSKLSPVLDGVSELRHSFDGSAADRYLLSQSSQPQSAATAPSAMFPYPGQHGPAHPAFSIGSPSRYMAHHPVITNGAYNSLLSNSSPQGYPTAGYPYPQQYGHSYQGAPFYQFSSTQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | T-box, brain, 1 |
| 别名 | T box brain protein 1, T box, brain, 1, T brain 1, T-box brain protein 1, TBR 1 |
| 计算分子量 | 682 aa, 74 kDa |
| 观测分子量 | 74 kDa |
| GenBank蛋白编号 | BC104844 |
| 基因名称 | TBR1 |
| Gene ID (NCBI) | 10716 |
| RRID | AB_2881925 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q16650 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TBR1 antibody 66564-1-Ig | Download protocol |
| IHC protocol for TBR1 antibody 66564-1-Ig | Download protocol |
| WB protocol for TBR1 antibody 66564-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis. | ||
EMBO J Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway. | ||
Biochem Biophys Res Commun Neuron-specific loss of Ppp6c induces neonatal death and decreases the number of cortical neurons and interneurons | ||
Nat Commun iPSC-derived models of PACS1 syndrome reveal transcriptional and functional deficits in neuron activity | ||
J Hazard Mater Effects of nanoplastic exposure during pregnancy and lactation on neurodevelopment of rat offspring |
