验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse testis tissue, mouse stomach tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TBPL1 Ready-To-Use IHC Kit. TBPL1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12258-1-AP targets TBPL1 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2880 Product name: Recombinant human TBPL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC017559 Sequence: MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TBP-like 1 |
| 别名 | 21 kDa TBP like protein, STUD, TBP like 1, TBP like factor, TBP like protein 1, TBP related factor 2, TBP related protein, TBPL1, TLF, TLP, TLP21, TRF2, TRP |
| 计算分子量 | 186 aa, 21 kDa |
| 观测分子量 | 21 kDa, 25 kDa |
| GenBank蛋白编号 | BC017559 |
| 基因名称 | TBPL1 |
| Gene ID (NCBI) | 9519 |
| RRID | AB_10642841 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62380 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| IP protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| WB protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesis | ||
Pharmacol Res Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway | ||
Cell Signal C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1. |
