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TBL1X Monoclonal antibody

TBL1X Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1A9A1

Cat No : 66955-1-Ig

Print datasheet

Synonyms

EBI, SMAP55, TBL1, TBL1X

验证数据展示

  • WB analysis of NIH/3T3 using 66955-1-Ig

    WB analysis of NIH/3T3 using 66955-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66955-1-Ig (TBL1X antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66955-1-Ig

    IHC staining of human breast cancer using 66955-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66955-1-Ig

    IHC staining of human breast cancer using 66955-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 66955-1-Ig

    IF Staining of HEK-293 using 66955-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TBL1X antibody (66955-1-Ig, Clone: 1A9A1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66955-1-Ig targets TBL1X in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen TBL1X fusion protein Ag5291 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name transducin (beta)-like 1X-linked
Synonyms EBI, SMAP55, TBL1, TBL1X
Calculated Molecular Weight 526 aa, 57 kDa
Observed Molecular Weight 57 kDa, 62 kDa
GenBank Accession NumberBC032708
Gene Symbol TBL1X
Gene ID (NCBI) 6907
RRIDAB_2882278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TBL1X antibody 66955-1-IgDownload protocol
IHC protocol for TBL1X antibody 66955-1-IgDownload protocol
IF protocol for TBL1X antibody 66955-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Arterioscler Thromb Vasc Biol

Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice

Authors - Mei Yang
View Article
humanWB

J Virol

Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.

Authors - Wenxiao Gong
View Article