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  • Featured Product
  • KD/KO Validated

TBL1X Polyclonal antibody

TBL1X Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 13540-1-AP

Print datasheet

Synonyms

EBI, SMAP55, TBL1, TBL1X

验证数据展示

  • IHC staining of mouse brain using 13540-1-AP

    IHC staining of mouse brain using 13540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13540-1-AP (TBL1X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse pancreas using 13540-1-AP

    WB analysis of mouse pancreas using 13540-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13540-1-AP (TBL1X antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13540-1-AP targets TBL1X in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA
Cited ApplicationsWB, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TBL1X fusion protein Ag4477 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transducin (beta)-like 1X-linked
Synonyms EBI, SMAP55, TBL1, TBL1X
Calculated Molecular Weight 526 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC032708
Gene Symbol TBL1X
Gene ID (NCBI) 6907
RRIDAB_2199783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBL1X is an essential subunit of the nuclear receptor corepressor (NCoR)-silencing mediator for retinoid and thyroid hormone receptors (SMRT) complex, the major TH receptor (TR) corepressor (CoR) involved in T3-regulated gene expression. TBL1X defects were recently associated with central hypothyroidism and hearing loss. (PMID: 30591955, PMID: 27603907)

实验方案

Product Specific Protocols
WB protocol for TBL1X antibody 13540-1-APDownload protocol
IHC protocol for TBL1X antibody 13540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Development

Setd5 is essential for mammalian development and the co-transcriptional regulation of histone acetylation.

Authors - Anna B Osipovich
View Article
humanWB,IP

Int J Biol Sci

TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.

Authors - Hongjuan Xu
  • KD Validated
View Article
mouseWB,CoIP,ChIP

Nat Commun

The Fgf/Erf/NCoR1/2 repressive axis controls trophoblast cell fate

Authors - Andreas Lackner
View Article
humanWB

Cell Chem Biol

HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation

Authors - Yufeng Xiao
View Article