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验证数据展示

  • WB analysis using 83686-3-RR

    WB analysis using 83686-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83686-3-RR

    WB analysis using 83686-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 83686-3-RR

    IHC staining of human placenta using 83686-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TBK1 antibody included
  • IHC staining of human placenta using 83686-3-RR

    IHC staining of human placenta using 83686-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TBK1 antibody included
  • IF Staining of MCF-7 using 83686-3-RR

    IF Staining of MCF-7 using 83686-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TBK1 antibody (83686-3-RR, Clone: 240769G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 83686-3-RR

    IF Staining of HeLa using 83686-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBK1 antibody (83686-3-RR, Clone: 240769G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TBK1 Recombinant monoclonal antibody

TBK1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83686-3-RR

产品说明书

CloneNo. 240769G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

240769G5, EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in4T1 cells, HCT 116 cells, HeLa cells, C6 cells, HSC-T6 cells, RAW 264.7 cells, mouse kidney tissue, HepG2 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy® TBK1 Ready-To-Use IHC Kit. TBK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83686-3-RR targets TBK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28894

Product name: Recombinant human TBK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 316-576 aa of BC034950

Sequence: LQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TANK-binding kinase 1
别名 240769G5, EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1
观测分子量84 kDa
GenBank蛋白编号BC034950
基因名称 TBK1
Gene ID (NCBI) 29110
RRIDAB_3671288
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHD2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

实验方案

Product Specific Protocols
IF protocol for TBK1 antibody 83686-3-RRDownload protocol
IHC protocol for TBK1 antibody 83686-3-RRDownload protocol
WB protocol for TBK1 antibody 83686-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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