验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, U2OS cells, HepG2 cells, Jurkat cells, LNCaP cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67211-1-Ig targets TBK1 in WB, IF, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | TBK1 fusion protein Ag28894 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TANK-binding kinase 1 |
| 别名 | Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1 |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC034950 |
| 基因名称 | TBK1 |
| Gene ID (NCBI) | 29110 |
| RRID | AB_2882504 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UHD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBK1 antibody 67211-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Chem Development of Orally Bioavailable Amidobenzimidazole Analogues Targeting Stimulator of Interferon Gene (STING) Receptor | ||
J Virol Porcine deltacoronavirus nsp5 antagonizes type I interferon signaling by cleaving IFIT3 | ||
Front Immunol Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1. | ||
Heliyon Metformin promotes cGAS/STING signaling pathway activation by blocking AKT phosphorylation in gastric cancer | ||
Biomed Pharmacother IFI16 promotes cervical cancer progression by upregulating PD-L1 in immunomicroenvironment through STING-TBK1-NF-kB pathway. | ||
Naunyn Schmiedebergs Arch Pharmacol Decursin protects against DSS-induced experimental colitis in mice by inhibiting the cGAS-STING signaling pathway |