验证数据展示

WB analysis using 28397-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28397-1-AP (TBK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBK1 antibody included
WB analysis using 28397-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28397-1-AP (TBK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 28397-1-AP
WB result of TBK1 antibody (28397-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and TBK1 knockout HeLa cells.
IHC staining of human stomach cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBK1 antibody included
IHC staining of human liver cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBK1 antibody included
IF Staining of MCF-7 using 28397-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TBK1 antibody (28397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 28397-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBK1 antibody (28397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IHC staining of human stomach cancer using 28397-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBK1 antibody included

Featured Product
KD/KO验证

TBK1 Polyclonal antibody

TBK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28397-1-AP

产品说明书

引用文献(79)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (7)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1, Serine/threonine-protein kinase TBK1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (79)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HT-1080 cells, HCT 116 cells, HeLa cells, HepG2 cells, U2OS cells
Positive IHC detected in	human stomach cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, HeLa cells

Planning an IHC experiment? We recommend our IHCeasy® TBK1 Ready-To-Use IHC Kit. TBK1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 78 publications below
IHC	See 3 publications below
IP	See 2 publications below

产品信息

28397-1-AP targets TBK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC, IP
经测试反应性	human
文献引用反应性	human, mouse, rat, pig, monkey, chicken, bovine, hamster
免疫原	CatNo: Ag28894 Product name: Recombinant human TBK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 316-576 aa of BC034950 Sequence: LQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	TANK-binding kinase 1
别名	EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1, Serine/threonine-protein kinase TBK1
观测分子量	84 kDa
GenBank蛋白编号	BC034950
基因名称	TBK1
Gene ID (NCBI)	29110
RRID	AB_2881132
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9UHD2
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

实验方案

Product Specific Protocols
IF protocol for TBK1 antibody 28397-1-AP	Download protocol
IHC protocol for TBK1 antibody 28397-1-AP	Download protocol
WB protocol for TBK1 antibody 28397-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Sci Adv Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation Authors - Wei Xiang View Article
mouse,human	WB	Mol Ther STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease Authors - Li Gao View Article
human	WB	Protein Cell Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response. Authors - Mi Li View Article
bovine	WB	J Anim Sci Biotechnol β-Hydroxybutyrate-induced mitochondrial DNA (mtDNA) release mediated innate inflammatory response in bovine mammary epithelial cells by inhibiting autophagy Authors - Yihui Huo View Article
human	WB	PLoS Pathog SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure Authors - Huilong Li View Article
human	WB	PLoS Pathog TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry Authors - Yifan Xie View Article

验证数据展示

WB analysis using 28397-1-AP

IHC staining of human liver cancer using 28397-1-AP

WB analysis using 28397-1-AP

WB analysis of HeLa using 28397-1-AP

IHC staining of human stomach cancer using 28397-1-AP

IHC staining of human liver cancer using 28397-1-AP

IF Staining of MCF-7 using 28397-1-AP

IF Staining of HeLa using 28397-1-AP

IHC staining of human stomach cancer using 28397-1-AP

TBK1 Polyclonal antibody

TBK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation

STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease

Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response.

β-Hydroxybutyrate-induced mitochondrial DNA (mtDNA) release mediated innate inflammatory response in bovine mammary epithelial cells by inhibiting autophagy

SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure

TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry