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验证数据展示

  • WB analysis using 20177-1-AP

    WB analysis using 20177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20177-1-AP (TBC1D9B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 20177-1-AP

    IP experiment of Jurkat using 20177-1-AP

    IP result of anti-TBC1D9B (IP:20177-1-AP, 4ug; Detection:20177-1-AP 1:1000) with Jurkat cells lysate 1920 ug.

  • IP experiment of HEK-293 using 20177-1-AP

    IP experiment of HEK-293 using 20177-1-AP

    IP result of anti-TBC1D9B (IP:20177-1-AP, 4ug; Detection:20177-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

TBC1D9B Polyclonal antibody

TBC1D9B Polyclonal Antibody for WB, IP, ELISA
Cat No. 20177-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

TBC1 domain family member 9B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inJurkat cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20177-1-AP targets TBC1D9B in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14019

Product name: Recombinant human TBC1D9B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1069-1250 aa of BC000701

Sequence: ARTGRKPRDCATEEDEPPAPELHQDAARELQPPAAGDPQAKAGGDTHLGTAPQESQVVVEGGSGEGQGSPSQLLSDDETKDDMSMSSYSVVSTGSLQCEDLADDTVLVGGEACSPTARIGGTVDTDWCISFEQILASILTESVLVNFFEKRVDIGLKIKDQKKVERQFSTASDHEQPGVSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 9B (with GRAM domain)
别名 TBC1 domain family member 9B
计算分子量 1250 aa, 140 kDa
观测分子量 141-150 kDa
GenBank蛋白编号BC000701
基因名称 TBC1D9B
Gene ID (NCBI) 23061
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ66K14
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1 domain family member 9B (TBC1D9B) a Rab11A GTPase-activating protein (Rab11A GAP), as a LMTK1 binding protein, and TBC1D9B inactivates Rab11A under the control of LMTK1A (PMID: 31628178). TBC1D9B is associated with endocytic vesicles, including those likely to contain active Rab11a (PMID: 25232007). TBC1D9B could also interact with other mammalian ATG8 homologues. TBC1D9B could be co-localized with LC3B on the autophagosome membranes (PMID: 30202024).

实验方案

Product Specific Protocols
WB protocol for TBC1D9B antibody 20177-1-APDownload protocol
IP protocol for TBC1D9B antibody 20177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols