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验证数据展示

  • WB analysis of human placenta using 20769-1-AP

    WB analysis of human placenta using 20769-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20769-1-AP

    WB analysis using 20769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of Neuro-2a using 20769-1-AP

    IP experiment of Neuro-2a using 20769-1-AP

    IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with Neuro-2a cells lysate 1440 ug.

  • IP experiment of HeLa using 20769-1-AP

    IP experiment of HeLa using 20769-1-AP

    IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with HeLa cells lysate 1200 ug.

TBC1D7 Polyclonal antibody

TBC1D7 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20769-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

PIG51, TBC1 domain family member 7, TBC1 domain family, member 7, TBC7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRamos cells, human placenta tissue, U266 cells, Neuro-2a cells
Positive IP detected inNeuro-2a cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20769-1-AP targets TBC1D7 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14756

Product name: Recombinant human TBC1D7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-293 aa of BC007054

Sequence: MTEDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVWKVLLGILPPHHESHAKVMMYRKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLPRSPSFPLEPDDEVFLAIAKAMEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLPKAFEQYLNLEDGRLLTHLRMCSAAPKLPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSAEKITKFLENIPQDSSDAIVSKAIDLWHKHCGTPVHSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 7
别名 PIG51, TBC1 domain family member 7, TBC1 domain family, member 7, TBC7
计算分子量 293 aa, 34 kDa
观测分子量 30 kDa
GenBank蛋白编号BC007054
基因名称 TBC1D7
Gene ID (NCBI) 51256
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0N9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D7 is a member of the TBC-domain containing protein family. The encoded protein functions as a subunit of the tuberous sclerosis TSC1-TSC2 complex which plays a role in the regulation of cellular growth and differentiation. Mutations in this gene have been associated with autosomal recessive megalencephaly.

实验方案

Product Specific Protocols
IP protocol for TBC1D7 antibody 20769-1-APDownload protocol
WB protocol for TBC1D7 antibody 20769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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