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验证数据展示

  • WB analysis using 68409-1-Ig

    WB analysis using 68409-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68409-1-Ig (TBC1D23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

TBC1D23 Monoclonal antibody

TBC1D23 Monoclonal Antibody for WB, ELISA
Cat No. 68409-1-Ig

产品说明书

CloneNo. 2A12A7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, pig

应用

WB, ELISA

别名

DKFZp667G062, FLJ11046, NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23, TBC1D23

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue
Planning an IHC experiment? We recommend our IHCeasy TBC1D23 Ready-To-Use IHC Kit. TBC1D23 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68409-1-Ig targets TBC1D23 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原

CatNo: Ag10727

Product name: Recombinant human TBC1D23 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-320 aa of BC010221

Sequence: MLQNPSEFAQSVKSLLEAQKQSIESGSIAGGEHLCFMGSGREEEDMYMNMVLAHFLQKNKEYVSIASGGFMALQQHLADINVDGPENGYGHWIASTSGSRSSINSVDGESPNGSSDRGMKSLVNKMTVALKTKSVNVREKVISFIENTSTPVDRHVSSSDRVGKPYRGVKPVFSIGDEEEYDTDEIDSSSMSDDDRKEVVNIQTWINKPDVKHHFPCKEVKESGHMFPSHLLVTATHMYCLREIVSRKGLAYIQSRQALNSVVKITSKKKHPELITFKYGNSSASGIEILAIERYLIPNAGDATKAIKQQIMKVLDALES

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 TBC1 domain family, member 23
别名 DKFZp667G062, FLJ11046, NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23, TBC1D23
计算分子量 699 aa, 78 kDa
观测分子量78 kDa
GenBank蛋白编号BC010221
基因名称 TBC1D23
Gene ID (NCBI) 55773
RRIDAB_3085126
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NUY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714)

实验方案

Product Specific Protocols
WB protocol for TBC1D23 antibody 68409-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols