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TBC1D22A Polyclonal antibody

TBC1D22A Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 18332-1-AP

Print datasheet

Synonyms

C22orf4, HSC79E021, TBC1 domain family member 22A, TBC1 domain family, member 22A, TBC1D22A

验证数据展示

  • WB analysis of HeLa using 18332-1-AP

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 18332-1-AP

    IHC staining of human cervical cancer using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 18332-1-AP

    IHC staining of human cervical cancer using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 18332-1-AP

    IF Staining of HeLa using 18332-1-AP

    Immunofluorescent analysis of HeLa cells using 18332-1-AP (TBC1D22A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells
Positive IHC detected inhuman cervical cancer tissue, human cervix tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18332-1-AP targets TBC1D22A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen TBC1D22A fusion protein Ag12942 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 22A
Synonyms C22orf4, HSC79E021, TBC1 domain family member 22A, TBC1 domain family, member 22A, TBC1D22A
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC001292
Gene Symbol TBC1D22A
Gene ID (NCBI) 25771
RRIDAB_2302953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TBC1D22A antibody 18332-1-APDownload protocol
IHC protocol for TBC1D22A antibody 18332-1-APDownload protocol
IF protocol for TBC1D22A antibody 18332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols