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验证数据展示

  • WB analysis using 17463-1-AP

    WB analysis using 17463-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17463-1-AP (TBC1D21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

TBC1D21 Polyclonal antibody

TBC1D21 Polyclonal Antibody for WB, ELISA
Cat No. 17463-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Male germ cell Rab GTPase-activating protein, MGCRABGAP, TBC1 domain family member 21, TBC1 domain family, member 21

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17463-1-AP targets TBC1D21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11301

Product name: Recombinant human TBC1D21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-336 aa of BC033516

Sequence: MTTLSPENSLSARQSASFILVKRKPPIDKTEWDSFFDESGHLAKSRDFICVNILERGLHPFVRTEAWKFLTGYFSWQSSQDERLTVDSMRRKNYKALCQMYEKIQPLLENLHRNFTETRNNIARDIQKIYDKDPLGNVLIDKKRLEKILLLSYVCNTQAEYQQGFHEMMMLFQLMVEHDHETFWLFQFFLQKTEHSCVINIGVAKNLDMLSTLITFLDPVFAEHLKGKGAGAVQSLFPWFCFCFQRAFKSFDDVWRLWEVLLTGKPCRNFQVLVAYSMLQMVREQVLQESMGGDDILLACNNLIDLDADELISAACVVYAELIQKDVPQTLKDFFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 21
别名 Male germ cell Rab GTPase-activating protein, MGCRABGAP, TBC1 domain family member 21, TBC1 domain family, member 21
计算分子量 336 aa, 39 kDa
观测分子量35 kDa
GenBank蛋白编号BC033516
基因名称 TBC1D21
Gene ID (NCBI) 161514
RRIDAB_3085524
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYX1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D21 genes (also known as male germ cell RAB GTPase-activating protein MGCRABGAP) are related to sterility, as determined through cDNA microarray testing of human testicular tissues exhibiting spermatogenic defects. TBC1D21 is a protein specifically expressed in the testes that exhibits specific localizations of elongating and elongated spermatids during mammalian spermiogenesis.

实验方案

Product Specific Protocols
WB protocol for TBC1D21 antibody 17463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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