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验证数据展示

  • WB analysis using 16464-1-AP

    WB analysis using 16464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 16464-1-AP

    WB analysis of A549 using 16464-1-AP

    WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

TBC1D20 Polyclonal antibody

TBC1D20 Polyclonal Antibody for WB, ELISA
Cat No. 16464-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C20orf140, TBC1 domain family member 20, TBC1 domain family, member 20, TBC1D20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16464-1-AP targets TBC1D20 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TBC1D20 fusion protein Ag9539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 20
别名 C20orf140, TBC1 domain family member 20, TBC1 domain family, member 20, TBC1D20
计算分子量 403 aa, 46 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC014983
基因名称 TBC1D20
Gene ID (NCBI) 128637
RRIDAB_3669245
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BZ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D20 belongs to a family of GTPase activator proteins of Rab-like small GTPases.TBC1D20 is an endoplasmic reticulum (ER) type II membrane protein with the TBC domain positioned in the cytosol. Depletion of this protein inhibits replication of the virus and HCV infection. Mutations in this gene are associated with Warburg micro syndrome 4.

实验方案

Product Specific Protocols
WB protocol for TBC1D20 antibody 16464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols