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TBC1D17 Polyclonal antibody

TBC1D17 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20482-1-AP

Print datasheet

Synonyms

TBC1 domain family member 17, TBC1 domain family, member 17, TBC1D17

验证数据展示

  • WB analysis using 20482-1-AP

    WB analysis using 20482-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 20482-1-AP

    IP experiment of Jurkat using 20482-1-AP

    IP result of anti-TBC1D17 (IP:20482-1-AP, 4ug; Detection:20482-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • IHC staining of human breast cancer using 20482-1-AP

    IHC staining of human breast cancer using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 20482-1-AP

    IHC staining of human breast cancer using 20482-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 20482-1-AP

    IF Staining of A549 using 20482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20482-1-AP (TBC1D17 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse thymus tissue, Jurkat cells, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20482-1-AP targets TBC1D17 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TBC1D17 fusion protein Ag14358 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 17
Synonyms TBC1 domain family member 17, TBC1 domain family, member 17, TBC1D17
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC003516
Gene Symbol TBC1D17
Gene ID (NCBI) 79735
RRIDAB_11232420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HA65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TBC1D17 antibody 20482-1-APDownload protocol
IHC protocol for TBC1D17 antibody 20482-1-APDownload protocol
IF protocol for TBC1D17 antibody 20482-1-APDownload protocol
IP protocol for TBC1D17 antibody 20482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols