验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, mouse spleen tissue, rat spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34255-1-AP targets TBC1D10C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag40608 Product name: Recombinant human TBC1D10C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-138 aa of BC062999 Sequence: SELSGPGPYRQADRYGFIGGSSAEPGPGHPPADLIRQREMKWVEMTSHWEKTMSRRYKKVKMQCRKGIPSALRARCWPLLCGAHVCQKNSPGTYQELAEAPGDPQWMETIGRD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TBC1 domain family, member 10C |
| 别名 | Carabin, TBC1 domain family member 10C |
| 计算分子量 | 446 aa, 50 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC062999 |
| 基因名称 | TBC1D10C |
| Gene ID (NCBI) | 374403 |
| RRID | AB_3743181 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8IV04 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBC1D10C (also known as Carabin or EPI64C) is a negative regulator of dual signaling pathways. It can inhibit the activity of small GTPase Ras through its N-terminal TBC domain, and also suppress the activity of calcineurin through its C-terminal binding site, thereby simultaneously negatively regulating the two key signaling pathways, Ras-MAPK and Ca²⁺-Calcineurin-NFAT.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBC1D10C antibody 34255-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
