验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66499 targets TAU in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag21926 Product name: Recombinant human TAU protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 5-208 aa of BC000558 Sequence: PRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2c |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein tau |
| 别名 | MAPT, 1E9A8, AT180, DDPAC, MAPTL |
| 计算分子量 | 37-46, 79-81 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC000558 |
| 基因名称 | TAU |
| Gene ID (NCBI) | 4137 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10636 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TAU antibody CL488-66499 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
