×
Use Able AI chat for product recommendations

验证数据展示

  • IHC staining of mouse liver using 33698-1-AP

    IHC staining of mouse liver using 33698-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33698-1-AP (TAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 33698-1-AP

    IHC staining of mouse liver using 33698-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33698-1-AP (TAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 33698-1-AP

    IF Staining of A549 using 33698-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TAT antibody (33698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TAT Polyclonal antibody

TAT Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 33698-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

EC:2.6.1.5, L-tyrosine:2-oxoglutarate aminotransferase, tyrosine aminotransferase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inmouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33698-1-AP targets TAT in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag39227

Product name: Recombinant human TAT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 294-454 aa of BC130534

Sequence: IHDRRDIFGNEIRDGLVKLSQRILGPCTIVQGALKSILCRTPGEFYHNTLSFLKSNADLCYGALAAIPGLRPVRPSGAMYLMVGIEMEHFPEFENDVEFTERLVAEQSVHCLPATCFEYPNFIRVVITVPEVMMLEACSRIQEFCEQHYHCAEGSQEECDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine aminotransferase
别名 EC:2.6.1.5, L-tyrosine:2-oxoglutarate aminotransferase, tyrosine aminotransferase
GenBank蛋白编号BC130534
基因名称 TAT
Gene ID (NCBI) 6898
RRIDAB_3743054
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP17735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosine aminotransferase, also known as tyrosine transaminase, is the first enzyme in the catabolic pathway of tyrosine. The gene of this transaminase is regulated by glucocorticoid hormones as well as via the cAMP pathway. TAT reversibly catalyses Tyr to 4-hydroxyphenylpyruvate (4-HPP), which is the substrate for pathways producing plastoquinone31, 32, tocopherols33, phenolic acid14, 28, and benzylisoquinoline alkaloid (BIA)34 in plants, although tyrosine is synthesised from 4-HPP by TAT in bacteria (28687763).

实验方案

Product Specific Protocols
IF protocol for TAT antibody 33698-1-APDownload protocol
IHC protocol for TAT antibody 33698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...