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验证数据展示

  • WB analysis of HEK-293 using 16739-1-AP

    WB analysis of HEK-293 using 16739-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 16739-1-AP

    IHC staining of human ovary using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary using 16739-1-AP

    IHC staining of human ovary using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 16739-1-AP

    IF Staining of HeLa using 16739-1-AP

    Immunofluorescent analysis of HeLa cells using 16739-1-AP (TASP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TASP1 Polyclonal antibody

TASP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16739-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Threonine aspartase subunit alpha, Threonine aspartase 1, Taspase-1, Taspase 1, EC:3.4.25.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary tissue, human kidney tissue, human liver tissue, human lung tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16739-1-AP targets TASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TASP1 fusion protein Ag9918 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 taspase, threonine aspartase, 1
别名 Threonine aspartase subunit alpha, Threonine aspartase 1, Taspase-1, Taspase 1, EC:3.4.25.-
计算分子量 420 aa, 44 kDa
观测分子量 50 kDa
GenBank蛋白编号BC025266
基因名称 TASP1
Gene ID (NCBI) 55617
RRIDAB_2201218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6P5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TASP1 antibody 16739-1-APDownload protocol
IHC protocol for TASP1 antibody 16739-1-APDownload protocol
IF protocol for TASP1 antibody 16739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Suleiman-El-Hattab syndrome: a histone modification disorder caused by TASP1 deficiency.

Authors - Korbinian M Riedhammer
  • KD Validated
View Article
humanWB,IHC

Cancer Manag Res

Overexpression of Taspase 1 Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma.

Authors - Jie Jiang
View Article