验证数据展示
经过测试的应用
| Positive WB detected in | mouse tongue tissue, rat tongue tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29344-1-AP targets TAS1R2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag29305 Product name: Recombinant human TAS1R2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 166-270 aa of NM_152232 Sequence: AISDELRDKVRFPALLRTTPSADHHIEAMVQLMLHFRWNWIIVLVSSDTYGRDNGQLLGERVARRDICIAFQETLPTLQPNQNMTSEERQRLVTIVDKLQQSTAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | taste receptor, type 1, member 2 |
| 别名 | G protein coupled receptor 71, GPR71, Sweet taste receptor T1R2, T1R2, TAS1R2, Taste receptor type 1 member 2, TR2 |
| 计算分子量 | 95 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | NM_152232 |
| 基因名称 | TAS1R2 |
| Gene ID (NCBI) | 80834 |
| RRID | AB_3086121 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TE23 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Taste receptor type 1, member 2 (TAS1R2) controls the oral sensing of sweetness. Genetic variations in TAS1R2 have been shown to be associated with differential sweetness intensity and varying carbohydrate intake levels among individuals (PMID:33930495).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAS1R2 antibody 29344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
