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验证数据展示

  • WB analysis of Raji using 28403-1-AP

    WB analysis of Raji using 28403-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 28403-1-AP (TAOK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TAOK3 Polyclonal antibody

TAOK3 Polyclonal Antibody for WB, ELISA
Cat No. 28403-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy TAOK3 Ready-To-Use IHC Kit. TAOK3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28403-1-AP targets TAOK3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag29158

Product name: Recombinant human TAOK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 366-430 aa of BC002756

Sequence: SMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQSVQSQALHYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAO kinase 3
别名 DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3
计算分子量 105 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC002756
基因名称 TAOK3
Gene ID (NCBI) 51347
RRIDAB_2918158
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2K8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAOK3 (Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18. It belongs to the STE Ser/Thr protein kinase family. TAOK3 acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. The protein can also be autophosphorylated (PMID:20949042).

实验方案

Product Specific Protocols
WB protocol for TAOK3 antibody 28403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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