验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
27065-1-AP targets TANK in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag25740 Product name: Recombinant human TANK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-119 aa of BC003388 Sequence: MDKNIGEQLNKAYEAFRQACMDRDSAVKELQQKTENYEQRIREQQEQLSLQQTIIDKLKSQLLLVNSTQDNNYGCVPLLEDSETRKNNLTLDQPQDKVISGIAREKLPKVDIASAESSI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TRAF family member-associated NFKB activator |
| 别名 | I TRAF, ITRAF, TANK, TRAF interacting protein, TRAF2 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC003388 |
| 基因名称 | TANK |
| Gene ID (NCBI) | 10010 |
| RRID | AB_2880738 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92844 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TANK antibody 27065-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function. | ||
Front Cell Infect Microbiol Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon. | ||
Int Immunopharmacol The effect of Astragaloside IV on ethanol-induced gastric mucosal injury in rats: Involvement of inflammation. | ||
J Mol Cell Biol TRIM14 promotes endothelial activation via activating NF-κB signaling pathway. |
