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  • Featured Product
  • KD/KO Validated

TALDO1 Polyclonal antibody

TALDO1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12376-1-AP

Print datasheet

Synonyms

TAL, TAL H, TALDO, TALDO1, TALDOR, TALH, Transaldolase, transaldolase 1

验证数据展示

  • WB analysis using 12376-1-AP

    WB analysis using 12376-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12376-1-AP (TALDO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 12376-1-AP

    IP experiment of COLO 320 using 12376-1-AP

    IP result of anti-TALDO1 (IP:12376-1-AP, 4ug; Detection:12376-1-AP 1:700) with COLO 320 cells lysate 3200 ug.

  • WB analysis of HEK-293 using 12376-1-AP

    WB analysis of HEK-293 using 12376-1-AP

    WB result of TALDO1 antibody (12376-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TALDO1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, COLO 320 cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy TALDO1 Ready-To-Use IHC Kit. TALDO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12376-1-AP targets TALDO1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TALDO1 fusion protein Ag3031 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transaldolase 1
Synonyms TAL, TAL H, TALDO, TALDO1, TALDOR, TALH, Transaldolase, transaldolase 1
Calculated Molecular Weight 337 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC009680
Gene Symbol TALDO1
Gene ID (NCBI) 6888
RRIDAB_2199712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP37837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TALDO1 belongs to the transaldolase family which contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP). Defects in TALDO1 are the cause of transaldolase 1 deficiency which results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.

实验方案

Product Specific Protocols
WB protocol for TALDO1 antibody 12376-1-APDownload protocol
IP protocol for TALDO1 antibody 12376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Science

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Authors - Issam Ben-Sahra
View Article
humanWB

Cancer Cell

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Authors - Yoichiro Mitsuishi
View Article
humanWB

Nat Commun

Synthetic lethality between HER2 and transaldolase in intrinsically resistant HER2-positive breast cancers.

Authors - Yi Ding
  • KD Validated
View Article
humanWB

Nat Metab

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.

Authors - Diem H Tran
View Article

J Biol Chem

Metabolic reprogramming driven by EZH2 inhibition depends on cell-matrix interactions

Authors - Teresa W-M Fan
View Article