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TAF9B Polyclonal antibody

TAF9B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 28713-1-AP

Print datasheet

Synonyms

DN 7, DN7, TAF9B, TAF9L, TAFII31L, TFIID 31

验证数据展示

  • WB analysis using 28713-1-AP

    WB analysis using 28713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28713-1-AP (TAF9B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 28713-1-AP

    IP experiment of HeLa using 28713-1-AP

    IP result of anti-TAF9B (IP:28713-1-AP, 4ug; Detection:28713-1-AP 1:500) with HeLa cells lysate 1880 ug.

  • IHC staining of mouse testis using 28713-1-AP

    IHC staining of mouse testis using 28713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28713-1-AP (TAF9B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28713-1-AP targets TAF9B in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen TAF9B fusion protein Ag29633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
Synonyms DN 7, DN7, TAF9B, TAF9L, TAFII31L, TFIID 31
Calculated Molecular Weight 28 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC010350
Gene Symbol TAF9B
Gene ID (NCBI) 51616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7, transcription initiation factor TFIID subunit 9-like, transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes, may have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. The calculated MW of TAF9B is 28 kDa, 28713-1-AP can detect a band around 30 kDa.

实验方案

Product Specific Protocols
WB protocol for TAF9B antibody 28713-1-APDownload protocol
IHC protocol for TAF9B antibody 28713-1-APDownload protocol
IP protocol for TAF9B antibody 28713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Nat Commun

Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation

Authors - Siyuan Sun
View Article