验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, human colon tissue, human skeletal muscle tissue, HEK-293 cells, human heart tissue, rat liver tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10544-1-AP targets TAF9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0833 Product name: Recombinant human TAF9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-172 aa of BC007426 Sequence: MLLPNILLTGTPGVGKTTLGKELASKSGLKYINVGDLAREEQLYDGYDEEYDCPILDEDRVVDELDNQMREGGVIVDYHGCDFFPERWFHIVFVLRTDTNVLYERLETRGYNEKKLTDNIQCEIFQVLYEEATASYKEEIVHQLPSNKPEELENNVDQILKWIEQWIKDHNS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa |
| 别名 | RNA polymerase II TBP-associated factor subunit G, STAF31/32, TAF2G, TAFII31, TAFII-31 |
| 计算分子量 | 28 kDa |
| 观测分子量 | 24-28 kDa |
| GenBank蛋白编号 | BC007426 |
| 基因名称 | TAF9 |
| Gene ID (NCBI) | 6880 |
| RRID | AB_2271416 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16594 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF9, also named as TAF2G, TAFII31, is a 264 amino acid protein, which belongs to the TAF9 family. TAF9 are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. TAF9 may have a role in gene regulation associated with apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TAF9 antibody 10544-1-AP | Download protocol |
| WB protocol for TAF9 antibody 10544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Ovarian Matrisome Dynamics and αvβ3-Mediated Regulation in Early Follicular Development. | ||
J Transl Med LncRNA938/ TAF9/TTK axis promotes EMT and serves as a therapeutic target in hepatoblastoma | ||
Mol Cell Proteomics Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation | ||
Front Pharmacol Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease.
| ||
Cancers (Basel) Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models. | ||
