验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse thymus tissue, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
15745-1-AP targets TAF6L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8180 Product name: Recombinant human TAF6L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC008785 Sequence: MSEREERRFVEIPRESVRLMAESTGLELSDEVAALLAEDVCYRLREATQNSSQFMKHTKRRKLTVEDFNRALRWSSVEAVCGYGSQEALPMRPAREGELYFPEDREVNLVELALATNIPKGCAETAVRVHVSYLDGKGNLAPQGSVPSAVSSLTDDLLKYYHQVTRAVLGDDPQLMKVALQDLQTNSKIGALLPYFVYVVSGVKSVSHDLEQLHRLLQVARSLFRNPHLCLGPYVRCLVGSVLYCVLEPLAASINPLNDHWTLRDGAALLLSHIFWTHGDLVSGLYQHILLSLQKILADPVRPLCCHYGAVVGLHALGWKAVERVLYPHLSTYWTNLQAVLDDYSVSNAQV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa |
| 别名 | PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L |
| 计算分子量 | 622 aa, 68 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC008785 |
| 基因名称 | TAF6L |
| Gene ID (NCBI) | 10629 |
| RRID | AB_2271394 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6J9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF6L (TBP-associated factor 6-like protein, also known as PAF65α) is a core component of the PCAF histone acetyltransferase complex and the STAGA transcriptional coactivation complex. This protein contains a characteristic histone folding domain (HFD) and is primarily located in the cell nucleus. As an epigenetic regulator, TAF6L works synergistically with TAF5L to promote the transcriptional elongation of RNA polymerase II by mediating H3K9ac deposition and MYC recruitment, thereby regulating embryonic stem cell pluripotency and somatic cell reprogramming.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF6L antibody 15745-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
