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验证数据展示

  • WB analysis of Jurkat using 19274-1-AP

    WB analysis of Jurkat using 19274-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19274-1-AP (TAF5L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 19274-1-AP

    IHC staining of human ovary cancer using 19274-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19274-1-AP (TAF5L antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TAF5L Polyclonal antibody

TAF5L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19274-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19274-1-AP targets TAF5L in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag5942

Product name: Recombinant human TAF5L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-325 aa of BC041094

Sequence: MKRVRTEQIQMAVSCYLKRRQYVDSDGPLKQGLRLSQTAEEMAANLTVQSESGCANIVSAAPCQAEPQQYEVQFGRLRNFLTDSDSQHSHEVMPLLYPLFVYLHLNLVQNSPKSTVESFYSRFHGMFLQNASQKDVIEQLQTTQTIQDILSNFKLRAFLDNKYVVRLQEDSYNYLIRYLQSDNNTALCKVLTLHIHLDVQPAKRTDYQLYASGSSSRSENNGLEPPDMPSPILQNEAALEVLQESIKRVKDGPPSLTTICFYAFYNTEQLLNTAEISPDSKLLAAGFDNSCIKLWSLRSKKLKSEPHQVDVSRIHLACDILEEEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
别名 TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC041094
基因名称 TAF5L
Gene ID (NCBI) 27097
RRIDAB_10642700
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75529
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF5L antibody 19274-1-APDownload protocol
IHC protocol for TAF5L antibody 19274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Nat Struct Mol Biol

A cohesin traffic pattern genetically linked to gene regulation

Authors - Anne-Laure Valton
View Article
humanWB

Sci Adv

The KAT module of the SAGA complex maintains the oncogenic gene expression program in MYCN-amplified neuroblastoma

Authors - Clare F Malone
View Article