验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, LNCaP cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33029-1-AP targets TAF4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38833 Product name: Recombinant human TAF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-590 aa of NM_003185.4 Sequence: QLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa |
| 别名 | RNA polymerase II TBP-associated factor subunit C, TAF2C, TAF2C1, TAF4A, TAFII-130 |
| 计算分子量 | 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | NM_003185.4 |
| 基因名称 | TAF4 |
| Gene ID (NCBI) | 6874 |
| RRID | AB_3742826 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O00268 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF4, also named as TAF2C, TAF2C1, TAF4A, TAFII130 and TAFII135, belongs to the TAF4 family. TAF4 makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4 potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF4 antibody 33029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
