验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, cells, SW480 cells, Y79 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
18901-1-AP targets TAF3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag13536 Product name: Recombinant human TAF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 443-721 aa of BC024661 Sequence: TKSGSTPLPLSGGTSSSDNSWTMDASIDEVVRKAKLGTPSNMPPNFPYISSPSVSPPTPEPLHKVYEEKTKLPSSVEVKKKLKKELKTKMKKKEKQRDREREKDKNKDKSKEKDKVKEKEKDKETGRETKYPWKEFLKEEEADPYKFKIKEFEDVDPKVKLKDGLVRKEKEKHKDKKKDREKGKKDKDKREKEKVKDKGREDKMKAPAPPLVLPPKELALPLFSPATASRVPAMLPSLLPVLPEKLFEEKEKVKEKEKKKDKKEKKKKKEKEKEKKEK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa |
| 别名 | TAFII-140, TAFII140, TAFII 140, TAF140, TAF(II)140 |
| 计算分子量 | 929 aa, 104 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC024661 |
| 基因名称 | TAF3 |
| Gene ID (NCBI) | 83860 |
| RRID | AB_2878567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VWG9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF3, also named as TAF140, is a 140 kDa subunit of Transcription initiation factor TFIID. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 has some isoforms with 727aa (~80 kDa), 622aa and 929aa (105 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF3 antibody 18901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
MBio A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression. | ||
Oncol Lett Identification of a novel FOXO3‑associated prognostic model in hepatocellular carcinoma |