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验证数据展示

  • WB analysis using 15362-1-AP

    WB analysis using 15362-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15362-1-AP (TAF1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TAF1A Polyclonal antibody

TAF1A Polyclonal Antibody for WB, ELISA
Cat No. 15362-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RAFI48, RNA polymerase I-specific TBP-associated factor 48 kDa, SL1, TAFI48, TATA box-binding protein-associated factor 1A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, A549 cells, HeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15362-1-AP targets TAF1A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8059

Product name: Recombinant human TAF1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-450 aa of BC013808

Sequence: PEIIWKLGSEILFYHPKSNMESFNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILINLIQAYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPGVWDPFVKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLISVLKILYQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAKYLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKGCRYFRYILKQDHQILGKKIKRMKRSVKKYSIVNPRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
别名 RAFI48, RNA polymerase I-specific TBP-associated factor 48 kDa, SL1, TAFI48, TATA box-binding protein-associated factor 1A
计算分子量 53 kDa
观测分子量48-52 kDa
GenBank蛋白编号BC013808
基因名称 TAF1A
Gene ID (NCBI) 9015
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAF1A, also named as TAFI48 and SL1, is a component of the transcription factor SL1/TIF-IB complex. TAF1A has two isoform with MW 40 kDa and 48-52 kDa.

实验方案

Product Specific Protocols
WB protocol for TAF1A antibody 15362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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