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验证数据展示

  • WB analysis of Jurkat using 25521-1-AP

    WB analysis of Jurkat using 25521-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25521-1-AP

    WB analysis of HEK-293 using 25521-1-AP

    WB result of TAF15 antibody (25521-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 25521-1-AP

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human colon cancer using 25521-1-AP

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human malignant melanoma using 25521-1-AP

    IHC staining of human malignant melanoma using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human cervical cancer using 25521-1-AP

    IHC staining of human cervical cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • Featured Product
  • KD/KO验证

TAF15 Polyclonal antibody

TAF15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25521-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TAF15 Ready-To-Use IHC Kit. TAF15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25521-1-AP targets TAF15 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
别名 TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68
计算分子量 62 kDa
观测分子量 65-70 kDa
GenBank蛋白编号NM_003487
基因名称 TAF15
Gene ID (NCBI) 8148
RRIDAB_2880116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92804
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF15 antibody 25521-1-APDownload protocol
IHC protocol for TAF15 antibody 25521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells

Authors - Rukiye Nar
View Article
humanWB,IP

F1000Res

A guide to selecting high-performing antibodies for TAF15 (UniProt ID: Q92804) for use in western blot, immunoprecipitation, and immunofluorescence

Authors - Vera Ruíz Moleón
View Article