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验证数据展示

  • IP experiment of HeLa using 16114-1-AP

    IP experiment of HeLa using 16114-1-AP

    IP result of anti-TAF11 (IP:16114-1-AP, 4ug; Detection:16114-1-AP 1:300) with HeLa cells lysate 1600 ug.

  • IHC staining of human colon cancer using 16114-1-AP

    IHC staining of human colon cancer using 16114-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 16114-1-AP

    WB analysis of HepG2 using 16114-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16114-1-AP

    IF Staining of HepG2 using 16114-1-AP

    Immunofluorescent analysis of HepG2 cells using 16114-1-AP (TAF11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TAF11 Polyclonal antibody

TAF11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TFIID subunit p30 beta, TAFII-28, TAFII28, TAFII 28, TAF2I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16114-1-AP targets TAF11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9039

Product name: Recombinant human TAF11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-211 aa of BC021972

Sequence: MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMPPLQPKHMREAVRRLKSKGQIPNSKHKKIIFF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa
别名 TFIID subunit p30 beta, TAFII-28, TAFII28, TAFII 28, TAF2I
计算分子量 211 aa, 23 kDa
观测分子量 28 kDa
GenBank蛋白编号BC021972
基因名称 TAF11
Gene ID (NCBI) 6882
RRIDAB_2878220
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAF11, also named as Transcription initiation factor TFIID 28 kDa subunit, is a 211 amino acid protein, which Belongs to the TAF11 family. TAF11 as a core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 . The calcualted molecular weight of TAF11 is 23 kDa, but modified TAF11 is about 28kDa. ( PMID: 10336414)

实验方案

Product Specific Protocols
WB protocol for TAF11 antibody 16114-1-APDownload protocol
IHC protocol for TAF11 antibody 16114-1-APDownload protocol
IF protocol for TAF11 antibody 16114-1-APDownload protocol
IP protocol for TAF11 antibody 16114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols