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验证数据展示

  • WB analysis of mouse testis using 20346-1-AP

    WB analysis of mouse testis using 20346-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20346-1-AP (TAF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20346-1-AP

    IHC staining of mouse testis using 20346-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20346-1-AP (TAF10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TAF10 Polyclonal antibody

TAF10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20346-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

STAF28, TAF(II)30, TAF10, TAF2A, TAF2H, TAFII 30, TAFII30

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20346-1-AP targets TAF10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag14153

Product name: Recombinant human TAF10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 70-218 aa of BC012088

Sequence: EPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
别名 STAF28, TAF(II)30, TAF10, TAF2A, TAF2H, TAFII 30, TAFII30
计算分子量 218 aa, 22 kDa
观测分子量 29 kDa
GenBank蛋白编号BC012088
基因名称 TAF10
Gene ID (NCBI) 6881
RRIDAB_2878678
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12962
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF10 antibody 20346-1-APDownload protocol
IHC protocol for TAF10 antibody 20346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Calcium saccharate/DUSP6 suppresses renal cell carcinoma glycolytic metabolism and boosts sunitinib efficacy via the ERK-AKT pathway

Authors - Huan Liu
View Article