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验证数据展示

  • WB analysis using 17367-1-AP

    WB analysis using 17367-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17367-1-AP (TADA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17367-1-AP

    IHC staining of human ovary cancer using 17367-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17367-1-AP (TADA2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

TADA2B Polyclonal antibody

TADA2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17367-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ADA2 beta, ADA2 like protein beta, ADA2(beta), ADA2B, ADA2-beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17367-1-AP targets TADA2B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag10783

Product name: Recombinant human TADA2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-420 aa of BC013164

Sequence: ERQRRKNIARDYNLVPAFLGKDKKEKEKALKRKITKEEKELRLKLRPLYQFMSCKEFDDLFENMHKEKMLRAKIRELQRYRRNGITKMEESAEYEAARHKREKRKENKNLAGSKRGKEDGKDSEFAAIENLPGFELLSDREKVLCSSLNLSPARYVTVKTIIIKDHLQKRQGIPSKSRLPSYLDKVLKKRILNFLTESGWISRDAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcriptional adaptor 2 (ADA2 homolog, yeast)-beta
别名 ADA2 beta, ADA2 like protein beta, ADA2(beta), ADA2B, ADA2-beta
计算分子量 420 aa, 48 kDa
观测分子量 55-62 kDa
GenBank蛋白编号BC013164
基因名称 TADA2B
Gene ID (NCBI) 93624
RRIDAB_2201049
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TADA2B antibody 17367-1-APDownload protocol
IHC protocol for TADA2B antibody 17367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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humanWB

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The KAT module of the SAGA complex maintains the oncogenic gene expression program in MYCN-amplified neuroblastoma

Authors - Clare F Malone
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