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TACR2 Polyclonal antibody

TACR2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25270-1-AP

Print datasheet

Synonyms

Neurokinin A receptor, NK 2 receptor, NK 2R, NK2R, NKNAR, SKR, Substance K receptor, TAC2R, tachykinin receptor 2, TACR2

验证数据展示

  • WB analysis of HeLa using 25270-1-AP

    WB analysis of HeLa using 25270-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25270-1-AP (TACR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 25270-1-AP

    FC experiment of PC-3 using 25270-1-AP

    1X10^6 PC-3 cells were stained with 0.2ug TACR2 antibody (25270-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive FC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25270-1-AP targets TACR2 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tachykinin receptor 2
Synonyms Neurokinin A receptor, NK 2 receptor, NK 2R, NK2R, NKNAR, SKR, Substance K receptor, TAC2R, tachykinin receptor 2, TACR2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberNM_001057
Gene Symbol TACR2
Gene ID (NCBI) 6865
RRIDAB_2879998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The tachykinins represent a family of peptide neurotransmitters including substance P, neurokinin A and neurokinin B, which act through the stimulation of tachykinin NK1, NK2 or NK3 receptors. NK2, also known as TACR2 (tachykinin receptor 2), is a receptor for the tachykinin neurokinin A (neuropeptide substance K). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

实验方案

Product Specific Protocols
WB protocol for TACR2 antibody 25270-1-APDownload protocol
FC protocol for TACR2 antibody 25270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer.

Authors - Wang Jianfeng
View Article
ratIHC

Mol Med Rep

Occurrence of substance P and neurokinin receptors during the early phase of spinal fusion.

Authors - Suiyi Wu
View Article