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验证数据展示

  • WB analysis of HeLa using 25270-1-AP

    WB analysis of HeLa using 25270-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25270-1-AP (TACR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 25270-1-AP

    FC experiment of HeLa using 25270-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TACR2 Polyclonal antibody (25270-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

TACR2 Polyclonal antibody

TACR2 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 25270-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, FC (Intra), ELISA and More (1)

别名

Neurokinin A receptor, NK 2 receptor, NK 2R, NK-2 receptor, NK2R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25270-1-AP targets TACR2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, rat
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tachykinin receptor 2
别名 Neurokinin A receptor, NK 2 receptor, NK 2R, NK-2 receptor, NK2R
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号NM_001057
基因名称 TACR2
Gene ID (NCBI) 6865
RRIDAB_2879998
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The tachykinins represent a family of peptide neurotransmitters including substance P, neurokinin A and neurokinin B, which act through the stimulation of tachykinin NK1, NK2 or NK3 receptors. NK2, also known as TACR2 (tachykinin receptor 2), is a receptor for the tachykinin neurokinin A (neuropeptide substance K). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

实验方案

Product Specific Protocols
WB protocol for TACR2 antibody 25270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer.

Authors - Wang Jianfeng
View Article
ratIHC

Mol Med Rep

Occurrence of substance P and neurokinin receptors during the early phase of spinal fusion.

Authors - Suiyi Wu
View Article