验证数据展示
经过测试的应用
| Positive WB detected in | MDCK cells, Raji cells, NIH/3T3 cells, mouse spleen tissue, mouse thymus, rat spleen tissue |
| Positive IP detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87915-1-RR targets Syntaxin 7 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag2975 Product name: Recombinant human STX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC011975 Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | syntaxin 7 |
| 别名 | Syntaxin-7, STX7 |
| 计算分子量 | 261 aa, 30 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC011975 |
| 基因名称 | Syntaxin 7 |
| Gene ID (NCBI) | 8417 |
| RRID | AB_3745823 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 7(STX7) is a SNARE protein localized to late endosomes and lysosomes, mediating vesicle fusion and intracellular trafficking. It plays essential roles in autophagy, pathogen degradation, and cholesterol transport through interactions with VAMP8 and Vti1b.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for Syntaxin 7 antibody 87915-1-RR | Download protocol |
| WB protocol for Syntaxin 7 antibody 87915-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
