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验证数据展示

  • WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1B antibody included
  • IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig ( Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1B antibody included
  • IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Syntaxin 1B Monoclonal antibody, PBS Only

Syntaxin 1B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66437-1-PBS

产品说明书

CloneNo. 2D3B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

Syntaxin 1A / Syntaxin 1B, Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1B Ready-To-Use IHC Kit. Syntaxin 1B primary antibody included.

产品信息

66437-1-PBS targets Syntaxin 1B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag10257

Product name: Recombinant human STX1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-263 aa of BC062298

Sequence: MKDRTQELRSAKDSDDEEEVVHVDRDHFMDEFFEQVEEIRGCIEKLSEDVEQVKKQHSAILAAPNPDEKTKQELEDLTADIKKTANKVRSKLKAIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMTEYNATQSKYRDRCKDRIQRQLEITGRTTTNEELEDMLESGKLAIFTDDIKMDSQMTKQALNEIETRHNEIIKLETSIRELHDMFVDMAMLVESQGEMIDRIEYNVEHSVDYVERAVSDTKKAVKYQSKARRK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 1B
别名 Syntaxin 1A / Syntaxin 1B, Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5
计算分子量 288 aa, 33 kDa
观测分子量 33 kDa, 55 kDa
GenBank蛋白编号BC062298
基因名称 STX1B
Gene ID (NCBI) 112755
RRIDAB_2881807
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP61266
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611). This antibody may react with both Syntaxin 1B and Syntaxin 1A.

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