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Conjugate
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验证数据展示

  • WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1A / Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Syntaxin 1A / Syntaxin 1B Monoclonal antibody, PBS Only

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66437-1-PBS

产品说明书

CloneNo. 2D3B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5, STX1B1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.

产品信息

66437-1-PBS targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Syntaxin 1A / Syntaxin 1B fusion protein Ag10257 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 1B
别名 Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5, STX1B1
计算分子量 288 aa, 33 kDa
观测分子量 33 kDa, 55 kDa
GenBank蛋白编号BC062298
基因名称 STX1B
Gene ID (NCBI) 112755
RRIDAB_2881807
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61266
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).