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验证数据展示

  • WB analysis using 27749-1-AP

    WB analysis using 27749-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27749-1-AP (Syndetin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27749-1-AP

    IP experiment of mouse brain using 27749-1-AP

    IP result of anti-Syndetin/CCDC132 (IP:27749-1-AP, 4ug; Detection:27749-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

  • IHC staining of human rectal cancer using 27749-1-AP

    IHC staining of human rectal cancer using 27749-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syndetin/CCDC132 antibody included
  • IHC staining of mouse brain using 27749-1-AP

    IHC staining of mouse brain using 27749-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syndetin/CCDC132 antibody included
  • IHC staining of human rectal cancer using 27749-1-AP

    IHC staining of human rectal cancer using 27749-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syndetin/CCDC132 antibody included
  • IHC staining of mouse brain using 27749-1-AP

    IHC staining of mouse brain using 27749-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syndetin/CCDC132 antibody included
  • IHC staining of mouse skeletal muscle using 27749-1-AP

    IHC staining of mouse skeletal muscle using 27749-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syndetin/CCDC132 antibody included

Syndetin/CCDC132 Polyclonal antibody

Syndetin/CCDC132 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27749-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

CCDC132, Syndetin, Coiled-coil domain-containing protein 132, VPS50

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman rectal cancer tissue, human placenta tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Syndetin/CCDC132 Ready-To-Use IHC Kit. Syndetin/CCDC132 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27749-1-AP targets Syndetin/CCDC132 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26944

Product name: Recombinant human CCDC132 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 337-593 aa of BC152424

Sequence: EWHEKHDNEDTASASEGSNMIGTEETNFDRGYIKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIISRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFMEQSRSPSVSPSKQPVSTSSKTVTLFEQYCSGGNPFEIQANHKDEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEELKRDYVDEQTGDGPVKSVSRETLKSRKKSDYSLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 132
别名 CCDC132, Syndetin, Coiled-coil domain-containing protein 132, VPS50
计算分子量 964 aa, 111 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC152424
基因名称 Syndetin/CCDC132
Gene ID (NCBI) 55610
RRIDAB_2880961
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JG6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syndetin, also known as CCDC132 or VPS50, is a critical component of the endosome-associated recycling protein (EARP) complex, which also includes ANG2/VPS51, VPS52, and VPS53 in a 1:1:1:1 ratio. The deduced 964-amino acid protein contains alpha-helical N- and C-terminal regions connected by a less structured middle region. CCDC132 is elevated in gastric cancer, where knockdown attenuates proliferation and tumorigenesis by facilitating DNA damage signaling, and its expression correlates with nodal metastasis and poor prognosis.

实验方案

Product Specific Protocols
IHC protocol for Syndetin/CCDC132 antibody 27749-1-APDownload protocol
IP protocol for Syndetin/CCDC132 antibody 27749-1-APDownload protocol
WB protocol for Syndetin/CCDC132 antibody 27749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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