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验证数据展示

  • IF Staining of SH-SY5Y using 67864-1-Ig

    IF Staining of SH-SY5Y using 67864-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 67864-1-Ig

    WB analysis using 67864-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67864-1-Ig

    WB analysis using 67864-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67864-1-Ig

    IHC staining of mouse brain using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of mouse brain using 67864-1-Ig

    IHC staining of mouse brain using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IF Staining of mouse pancreas using 67864-1-Ig

    IF Staining of mouse pancreas using 67864-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human pituitary adenoma using 67864-1-Ig

    IHC staining of human pituitary adenoma using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pituitary adenoma using 67864-1-Ig

    IHC staining of human pituitary adenoma using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 67864-1-Ig

    IHC staining of human appendicitis using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 67864-1-Ig

    IHC staining of human colorectal cancer using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pituitary adenoma using 67864-1-Ig

    IHC staining of human pituitary adenoma using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 67864-1-Ig

    IHC staining of human appendicitis using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human brain using 67864-1-Ig

    IHC staining of human brain using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human pituitary adenoma using 67864-1-Ig

    IHC staining of human pituitary adenoma using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human pituitary adenoma using 67864-1-Ig

    IHC staining of human pituitary adenoma using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of rat brain using 67864-1-Ig

    IHC staining of rat brain using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of mouse cerebellum using 67864-1-Ig

    IHC staining of mouse cerebellum using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of rat cerebellum using 67864-1-Ig

    IHC staining of rat cerebellum using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of mouse brain using 67864-1-Ig

    IHC staining of mouse brain using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human gliomas using 67864-1-Ig

    IHC staining of human gliomas using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human rectal cancer using 67864-1-Ig

    IHC staining of human rectal cancer using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of rat pancreas using 67864-1-Ig

    IHC staining of rat pancreas using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human pancreas using 67864-1-Ig

    IHC staining of human pancreas using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human stomach cancer using 67864-1-Ig

    IHC staining of human stomach cancer using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human colorectal cancer using 67864-1-Ig

    IHC staining of human colorectal cancer using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human pancreas cancer using 67864-1-Ig

    IHC staining of human pancreas cancer using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of human pituitary adenoma using 67864-1-Ig

    IHC staining of human pituitary adenoma using 67864-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IF Staining of rat brain using 67864-1-Ig

    IF Staining of rat brain using 67864-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for WB, IHC, IHC-Autostainer, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 67864-1-Ig

产品说明书

CloneNo. 4E12C4
引用文献(54)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IHC-Autostainer, IF/ICC, IF-P, IF-Fro, ELISA and More (1)

别名

SYP, 4E12C4, MRX96, SYN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, pig brain tissue, mouse brain tissue, rat brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue
Positive IHC-Autostainer detected inhuman pituitary adenoma tissue, human colorectal cancer tissue, human appendicitis tissue
Positive IHC detected inmouse brain tissue, human appendicitis tissue, human stomach cancer tissue, mouse cerebellum tissue, human pancreas tissue, human pituitary adenoma tissue, human rectal cancer tissue, rat cerebellum tissue, rat pancreas tissue, rat brain tissue, human colorectal cancer tissue, human gliomas tissue, human pancreas cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue, mouse brain tissue
Positive IF-Fro detected inrat brain tissue
Positive IF/ICC detected inSH-SY5Y cells, PC-12 cells
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67864-1-Ig targets Synaptophysin in WB, IHC, IHC-Autostainer, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IHC-Autostainer, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human, mouse, rat, pig
免疫原

CatNo: Ag11803

Product name: Recombinant human Synaptophysin; SYP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 50-313 aa of BC064550

Sequence: ELQLSVDCANKTESDLSIEVEFEYPFRLHQVYFDAPTCRGGTTKVFLVGDYSSSAEFFVTVAVFAFLYSMGALATYIFLQNKYRENNKGPMLDFLATAVFAFMWLVSSSAWAKGLSDVKMATDPENIIKEMPVCRQTGNTCKELRDLVTSGLNTSVVFGFLNLVLWVGNLWFVFKETGWAAPFLRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPAGSGGSGYGPQGDYGQQGYGPQGAPTSFSNQM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptophysin
别名 SYP, 4E12C4, MRX96, SYN
计算分子量 313 aa, 34 kDa
观测分子量38 kDa
GenBank蛋白编号BC064550
基因名称 Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2918622
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

实验方案

Product Specific Protocols
IF protocol for Synaptophysin antibody 67864-1-IgDownload protocol
IHC protocol for Synaptophysin antibody 67864-1-IgDownload protocol
WB protocol for Synaptophysin antibody 67864-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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