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验证数据展示

  • WB analysis using 29734-1-AP

    WB analysis using 29734-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29734-1-AP (SYNGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29734-1-AP

    WB analysis using 29734-1-AP

    Unboiled rat brain tissue was subjected to SDS PAGE followed by western blot with 29734-1-AP (SYNGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29734-1-AP

    IHC staining of mouse brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29734-1-AP

    IHC staining of mouse brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 29734-1-AP

    IHC staining of rat brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 29734-1-AP

    IHC staining of rat brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYNGR1 Polyclonal antibody

SYNGR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29734-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Synaptogyrin-1, synaptogyrin 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inunboiled rat brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29734-1-AP targets SYNGR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31214

Product name: Recombinant human SYNGR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-92 aa of BC000731

Sequence: MEGGAYGAGKAGGAFDPYTLVRQPHTILRVVSWLFSIVVFGSIVNEGYLNSASEGEEFCIYNRNPNACSYGVAVGVLAFLTCLLYLALDVYF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptogyrin 1
别名 Synaptogyrin-1, synaptogyrin 1
计算分子量 25 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC000731
基因名称 SYNGR1
Gene ID (NCBI) 9145
RRIDAB_2923601
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43759
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

实验方案

Product Specific Protocols
IHC protocol for SYNGR1 antibody 29734-1-APDownload protocol
WB protocol for SYNGR1 antibody 29734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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