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验证数据展示

  • IHC staining of mouse lung using 86322-1-RR

    IHC staining of mouse lung using 86322-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 86322-1-RR (Surfactant Protein A antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SFTPA1 antibody included
  • IHC staining of mouse lung using 86322-1-RR

    IHC staining of mouse lung using 86322-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 86322-1-RR (Surfactant Protein A antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SFTPA1 antibody included
  • IF Staining of human lung using 86322-1-RR

    IF Staining of human lung using 86322-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Surfactant Protein A antibody (86322-1-RR, Clone: 250912G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human lung using 86322-1-RR

    IF Staining of human lung using 86322-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Surfactant Protein A antibody (86322-1-RR, Clone: 250912G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Surfactant Protein A Recombinant monoclonal antibody

Surfactant Protein A Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 86322-1-RR

产品说明书

CloneNo. 250912G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

SFTPA1, SFTPA2, 35 kDa pulmonary surfactant-associated protein, COLEC4, Collectin 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung tissue, rat lung tissue, mouse lung tissue
Planning an IHC experiment? We recommend our IHCeasy SFTPA1 Ready-To-Use IHC Kit. SFTPA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86322-1-RR targets Surfactant Protein A in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2417

Product name: Recombinant human SFTPA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-248 aa of BC026229

Sequence: MTVGEKVFSSNGQSITFDAIQEACARAGGRIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRNCLYSRLTICEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 surfactant protein A1
别名 SFTPA1, SFTPA2, 35 kDa pulmonary surfactant-associated protein, COLEC4, Collectin 4
计算分子量 248 aa, 26 kDa
GenBank蛋白编号BC026229
基因名称 Surfactant Protein A
Gene ID (NCBI) 653509
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWL2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

实验方案

Product Specific Protocols
IF protocol for Surfactant Protein A antibody 86322-1-RRDownload protocol
IHC protocol for Surfactant Protein A antibody 86322-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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