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验证数据展示

  • WB analysis using 27524-1-AP

    WB analysis using 27524-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27524-1-AP (Supervillin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27524-1-AP

    IHC staining of mouse heart using 27524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 27524-1-AP

    IHC staining of mouse skeletal muscle using 27524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 27524-1-AP

    IHC staining of mouse skeletal muscle using 27524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Supervillin Polyclonal antibody

Supervillin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27524-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SVIL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27524-1-AP targets Supervillin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26196

Product name: Recombinant human Supervillin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1679-1787 aa of NM_003174

Sequence: MFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 supervillin
别名 SVIL
计算分子量 248 kDa
观测分子量 205 kDa
GenBank蛋白编号NM_003174
基因名称 SVIL
Gene ID (NCBI) 6840
RRIDAB_3669607
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95425
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Supervillin (SVIL) belongs to the villin/gelsolin superfamily of actin-binding proteins involved in many cellular processes. A non-muscle-specific form of Supervillin activates androgen receptor activity. Supervillin another isoform, called Archvillin, is expressed in skeletal and cardiac muscle tissue where it plays a role in both muscle fiber structure and signaling. Mutations in Supervillin are associated with myofibrillar Myopathy10 (MFM10), which causes myopathy with myofibrillar disorganization and autophagic vacuoles(PMID: 9867483, 32779703).

实验方案

Product Specific Protocols
IHC protocol for Supervillin antibody 27524-1-APDownload protocol
WB protocol for Supervillin antibody 27524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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