验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293T cells |
| Positive IP detected in | Transfected HEK-293F cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
85798-1-RR targets Strep Tag II in WB, IF/ICC, IP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | yeast |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Strep Tag II |
| 别名 | Strep, Strep-Tag, Strep-Tag II |
| 计算分子量 | 1 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3744381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Strep Tag II |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Strep-Tag II consists of 8 amino acids with the sequence of Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. Compared with Strep-Tag, Strep-Tag II has a higher binding affinity with streptavidin and can be constructed at the N-terminus or C-terminus of the target protein, providing greater flexibility.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Strep Tag II antibody 85798-1-RR | Download protocol |
| IP protocol for Strep Tag II antibody 85798-1-RR | Download protocol |
| WB protocol for Strep Tag II antibody 85798-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
