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验证数据展示

  • WB analysis using 30287-1-AP

    WB analysis using 30287-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30287-1-AP

    WB analysis using 30287-1-AP

    rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 30287-1-AP

    IP experiment of mouse testis using 30287-1-AP

    IP result of anti-Spag8 (IP:30287-1-AP, 4ug; Detection:30287-1-AP 1:3000) with mouse testis tissue lysate 1120 ug.

  • IHC staining of mouse testis using 30287-1-AP

    IHC staining of mouse testis using 30287-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Spag8 Polyclonal antibody

Spag8 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 30287-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

rat, mouse

应用

WB, IP, IHC, ELISA

别名

MH SPAG8, Spag8, sperm associated antigen 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30287-1-AP targets Spag8 in WB, IP, IHC, ELISA applications and shows reactivity with rat, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 rat, mouse
免疫原 Spag8 fusion protein Ag31603 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 8
别名 MH SPAG8, Spag8, sperm associated antigen 8
观测分子量60-70 kDa
GenBank蛋白编号BC150868
基因名称 Spag8
Gene ID (NCBI) 433700
RRIDAB_3086286
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3V0Q6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Spag8 antibody 30287-1-APDownload protocol
IHC protocol for Spag8 antibody 30287-1-APDownload protocol
IP protocol for Spag8 antibody 30287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols