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Spag8 Polyclonal antibody

Spag8 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 30287-1-AP

Print datasheet

Synonyms

MH SPAG8, Spag8, sperm associated antigen 8

验证数据展示

  • WB analysis using 30287-1-AP

    WB analysis using 30287-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30287-1-AP

    WB analysis using 30287-1-AP

    rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 30287-1-AP

    IP experiment of mouse testis using 30287-1-AP

    IP result of anti-Spag8 (IP:30287-1-AP, 4ug; Detection:30287-1-AP 1:3000) with mouse testis tissue lysate 1120 ug.

  • IHC staining of mouse testis using 30287-1-AP

    IHC staining of mouse testis using 30287-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30287-1-AP targets Spag8 in WB, IP, IHC, ELISA applications and shows reactivity with rat, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity rat, mouse
Immunogen Spag8 fusion protein Ag31603 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 8
Synonyms MH SPAG8, Spag8, sperm associated antigen 8
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC150868
Gene Symbol Spag8
Gene ID (NCBI) 433700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3V0Q6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Spag8 antibody 30287-1-APDownload protocol
IHC protocol for Spag8 antibody 30287-1-APDownload protocol
IP protocol for Spag8 antibody 30287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols