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验证数据展示

  • WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

    WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

    WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Sestrin 2 Monoclonal antibody, PBS Only

Sestrin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66297-1-PBS

产品说明书

CloneNo. 1A1A4

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66297-1-PBS targets Sestrin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig, rat
免疫原

CatNo: Ag20638

Product name: Recombinant human Sestrin2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 132-480 aa of BC013304

Sequence: HMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sestrin 2
别名 HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC013304
基因名称 SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_2881680
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP58004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

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