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验证数据展示

  • WB analysis using 31813-1-AP

    WB analysis using 31813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31813-1-AP (Serum amyloid P component antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31813-1-AP

    IHC staining of human stomach cancer using 31813-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31813-1-AP (Serum amyloid P component antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31813-1-AP

    IHC staining of mouse liver using 31813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31813-1-AP (Serum amyloid P component antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31813-1-AP

    IHC staining of mouse liver using 31813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31813-1-AP (Serum amyloid P component antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 31813-1-AP

    IF Staining of mouse liver using 31813-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Serum amyloid P component antibody (31813-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 31813-1-AP

    IF Staining of mouse liver using 31813-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Serum amyloid P component antibody (31813-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Serum amyloid P component Polyclonal antibody

Serum amyloid P component Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31813-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

APCS, SAP, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein, amyloid P component, serum

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman stomach cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31813-1-AP targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg0528

Product name: Recombinant Human APCS protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 20-223 aa of BC007058

Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amyloid P component, serum
别名 APCS, SAP, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein, amyloid P component, serum
计算分子量 223 aa, 25 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC007058
基因名称 Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_3670122
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP02743
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

实验方案

Product Specific Protocols
IF protocol for Serum amyloid P component antibody 31813-1-APDownload protocol
IHC protocol for Serum amyloid P component antibody 31813-1-APDownload protocol
WB protocol for Serum amyloid P component antibody 31813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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